Abstract

Holographic microscopy is an emerging biological technique that provides amplitude and quantitative phase imaging, though the contrast provided by many cell types and organelles is low, and until now no dyes were known that increased contrast. Here we show that the metallocorrole Ga(tpfc)${({\mathrm{SO}}_3\mathrm{H})}_2$, which has a strong Soret band absorption, increases contrast in both amplitude and phase and facilitates tracking of Escherichia coli with minimal toxicity. The change in phase contrast may be calculated from the dye-absorbance spectrum using the Kramers–Kronig relations, and represents a general principle that may be applied to any dye or cell type. This enables the use of holographic microscopy for all applications in which specific labeling is desired.

© 2015 Optical Society of America

Using the Gouy phase anomaly to localize and track bacteria in digital holographic microscopy 4D images

True Gibson, Manuel Bedrossian, Eugene Serabyn, Chris Lindensmith, and Jay L. Nadeau
J. Opt. Soc. Am. A 38(2) A11-A18 (2021)

Phase-shifting by means of an electronically tunable lens: quantitative phase imaging of biological specimens with digital holographic microscopy

Carlos Trujillo, Ana Doblas, Genaro Saavedra, Manuel Martínez-Corral, and Jorge García-Sucerquia
Opt. Lett. 41(7) 1416-1419 (2016)

Tomographic phase microscopy with 180° rotation of live cells in suspension by holographic optical tweezers

Mor Habaza, Barak Gilboa, Yael Roichman, and Natan T. Shaked
Opt. Lett. 40(8) 1881-1884 (2015)

Lens-free spectral light-field fusion microscopy for contrast- and resolution-enhanced imaging of biological specimens

Farnoud Kazemzadeh, Chao Jin, Sara Molladavoodi, Yu Mei, Monica B. Emelko, Maud B. Gorbet, and Alexander Wong
Opt. Lett. 40(16) 3862-3865 (2015)

Detectability of unresolved particles in off-axis digital holographic microscopy

Nikki Johnston, Megan Marie Dubay, Eugene Serabyn, and Jay L. Nadeau
Appl. Opt. 63(7) B114-B125 (2024)