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Volumetric structured illumination microscopy enabled by a tunable-focus lens

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Abstract

We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.

© 2015 Optical Society of America

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Supplementary Material (1)

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Visualization 1: MP4 (12251 KB)      Raw image stacks

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