Abstract
We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.
© 2015 Optical Society of America
Full Article | PDF ArticleMore Like This
Baris N. Ozbay, Justin T. Losacco, Robert Cormack, Richard Weir, Victor M. Bright, Juliet T. Gopinath, Diego Restrepo, and Emily A. Gibson
Opt. Lett. 40(11) 2553-2556 (2015)
Po-Hao Wang, Vijay Raj Singh, Jau-Min Wong, Kung-Bin Sung, and Yuan Luo
Opt. Lett. 42(2) 346-349 (2017)
Omar E. Olarte, Jordi Andilla, David Artigas, and Pablo Loza-Alvarez
Optica 2(8) 702-705 (2015)