Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Precise multi-emitter localization method for fast super-resolution imaging

Not Accessible

Your library or personal account may give you access

Abstract

We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solutions give the maximum likelihood estimator of multi-emitter positions. We compare the performance of our simultaneous localization analysis with the conventional single-molecule analysis for simulated images and show that our method can improve the time-resolution of super-resolution microscopy by an order of magnitude. In particular, we derive the information-theoretic bound on time resolution of localization-based super-resolution microscopy and demonstrate that the bound can be asymptotically attained by our analysis.

© 2015 Optical Society of America

Full Article  |  PDF Article
More Like This
Fast two-dimensional super-resolution image reconstruction algorithm for ultra-high emitter density

Jiaqing Huang, Kristyn Gumpper, Yuejie Chi, Mingzhai Sun, and Jianjie Ma
Opt. Lett. 40(13) 2989-2992 (2015)

Ultra-fast, high-precision image analysis for localization-based super resolution microscopy

Tingwei Quan, Pengcheng Li, Fan Long, Shaoqun Zeng, Qingming Luo, Per Niklas Hedde, Gerd Ulrich Nienhaus, and Zhen-Li Huang
Opt. Express 18(11) 11867-11876 (2010)

Tuning axial and lateral localization precision in 3D super-resolution microscopy with variable astigmatism

Rajdeep Chowdhury, Abhishek Sau, Jerry Chao, Ankith Sharma, and Siegfried M. Musser
Opt. Lett. 47(21) 5727-5730 (2022)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved