Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Improved lateral resolution with an annular vortex depletion beam in STED microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We report on the experimental demonstration of improved lateral resolution in stimulated emission depletion (STED) microscopy using an annular depletion beam configuration. A tight and finely tuned doughnut focal spot can be created by annular vortex illumination. Its application in STED microscopy for enhanced lateral resolution is systematically investigated by imaging 40 nm fluorescent beads. An improved resolution with more than 20% reduced effective point spread function of the imaging system determined by the full width at half-maximum compared to that of the conventional STED is achieved. The proposed scheme also demonstrates its resolving capability for biological samples. The principle holds great potential in the research fields of biological superresolution imaging as well as STED-based nanolithography and high-density optical data storage.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Coherent-hybrid STED: high contrast sub-diffraction imaging using a bi-vortex depletion beam

António Pereira, Mafalda Sousa, Ana C. Almeida, Luísa T. Ferreira, Ana Rita Costa, Marco Novais-Cruz, Cristina Ferrás, Mónica Mendes Sousa, Paula Sampaio, Michael Belsley, and Helder Maiato
Opt. Express 27(6) 8092-8111 (2019)

Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy

Shaocong Liu, Zhimin Zhang, Yubing Han, Lu Yang, Cuifang Kuang, and Xu Liu
Opt. Lett. 46(13) 3304-3307 (2021)

Precise background subtraction in stimulated emission double depletion nanoscopy

Peng Gao and G. Ulrich Nienhaus
Opt. Lett. 42(4) 831-834 (2017)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved