Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Simultaneous two-color stimulated Raman scattering microscopy by adding a fiber amplifier to a 2 ps OPO-based SRS microscope

Abstract

Stimulated Raman scattering (SRS) microscopy is a label-free chemical imaging technique. Two-color imaging is often necessary to determine the distribution of chemical species in SRS microscopy. Current multi-color SRS imaging methods involve complicated instrumentation or longer data acquisition time or are limited to transmission imaging. In this Letter, we show that by adding a simple fiber amplifier to a 2 ps laser source and optical-parametric-oscillator-based SRS setup, one can achieve simultaneous two-color or frequency modulation SRS microscopy. The fiber amplifier can generate a wavelength tunable laser of ±10nm around the Stokes laser wavelength at 1031 nm with average power greater than 200 mW. In vivo and ex vivo lipid–protein imaging of mouse brain and skin is demonstrated. To further demonstrate the potential of this technique in high-speed in vivo imaging, white blood cells in a blood stream are imaged in a live mouse.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging

Ruoyu He, Yongkui Xu, Lili Zhang, Shenghong Ma, Xu Wang, Dan Ye, and Minbiao Ji
Optica 4(1) 44-47 (2017)

Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

Ruoyu He, Zhiping Liu, Yongkui Xu, Wei Huang, Hong Ma, and Minbiao Ji
Opt. Lett. 42(4) 659-662 (2017)

High-speed stimulated hyperspectral Raman imaging using rapid acousto-optic delay lines

Mohammed S. Alshaykh, Chien-Sheng Liao, Oscar E. Sandoval, Gregory Gitzinger, Nicolas Forget, Daniel E. Leaird, Ji-Xin Cheng, and Andrew M. Weiner
Opt. Lett. 42(8) 1548-1551 (2017)

Supplementary Material (2)

NameDescription
Visualization 1: AVI (3873 KB)      Epi-SRS in vivo mouse skin imaging.
Visualization 2: AVI (4168 KB)      Epi-SRS ex vivo mouse brain imaging.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved