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Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, and Jerome Mertz

Abstract

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

© 2017 Optical Society of America

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