Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Single-wavelength excited photoacoustic-fluorescence microscopy for in vivo pH mapping

Not Accessible

Your library or personal account may give you access

Abstract

The balance between radiative and non-radiative decay is affected by various factors at the excitation state. A simultaneous acquisition of the fluorescence and photoacoustic signals from an appropriate probe provides an efficient, high-resolution means to monitor such a balance in a biological target, and thus may render its physiological information. Acidity plays an important role in tissue physiology. Here, we report an integrated photoacoustic-fluorescence microscopy (PA-FLM) for high-resolution (<3μm) image mapping of interstitial pH by detecting the shift in the signal balance of a pH-sensitive probe. The hypothesis and the technical feasibility are validated with an in vivo tumor model. The results show that the technique can effectively monitor pH changes within the range of biological acidity and are independent of the excitation source fluctuation and local probe concentration. We thus propose that, with further research and selection of proper probes, PA-FLM may provide a potential alternative for monitoring tissue physiology.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Photoacoustic-fluorescence microendoscopy in vivo

Wenzhao Yang, Jiasheng Zhou, Weihao Shao, Myeongsu Seong, Pengbo He, Zhanhong Ye, Zhendong Guo, Lili Jing, and Sung-Liang Chen
Opt. Lett. 46(10) 2340-2343 (2021)

Dual-modal imaging with non-contact photoacoustic microscopy and fluorescence microscopy

Jiasheng Zhou, Wei Wang, Lili Jing, and Sung-Liang Chen
Opt. Lett. 46(5) 997-1000 (2021)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved