Abstract

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells.

© 2018 Optical Society of America

Simultaneous two-wavelength phase unwrapping using an external module for multiplexing off-axis holography

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Six-pack off-axis holography

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Flipping interferometry with doubled imaging area

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