Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling

Not Accessible

Your library or personal account may give you access

Abstract

High-speed imaging is of the utmost importance for video-rate live cell investigations or to study extended sample areas at sufficient spatial resolution within reasonable time scales. Improving the speed of single-focus stimulated Raman scattering (SRS) microscopy is ultimately restricted by the sample’s damage threshold and the shot noise of the demodulated laser source. To overcome this limitation, we present a dual-focus SRS approach modulating the pump laser for each focus at a distinct frequency. The corresponding probe beams are detected each by a photodiode and demodulated individually by two separate lock-in units to avoid inter-focal cross-talk. Two laterally or axially displaced images as well as hyperspectral SRS images can be obtained simultaneously within the field of view of the objective lens. The modular implementation presented here can be extended to multiple foci by using multi-channel acousto-optics modulators in combination with multi-channel lock-in amplifiers.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies

Sandro Heuke, Barbara Sarri, Xavier Audier, and Hervé Rigneault
Opt. Lett. 43(15) 3582-3585 (2018)

Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source

Fisseha Bekele Legesse, Tobias Meyer, Sandro Heuke, Thomas Gottschall, Torbjörn Pascher, Jens Limpert, Andreas Tünnermann, Michael Schmitt, and Jürgen Popp
Opt. Lett. 42(2) 183-186 (2017)

Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging

Zi Wang, Wei Zheng, and Zhiwei Huang
Opt. Lett. 41(17) 3960-3963 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved