Abstract
We propose a numerical and totally automatic phase aberration compensation method in digital holographic microscopy. The phase aberrations are extracted in a nonlinear optimization procedure in which the phase variation of the reconstructed object wave is minimized. Not only phase curvature but also high-order aberrations could be corrected without extra devices. The correction is directly carried out with the wrapped phase map, which is not affected by phase unwrapping or fitting errors. Numerical simulation proves that the proposed method is more accurate than the conventional surface fitting method without selecting a cell-free background. Experimental results demonstrate the availability of the proposed method in real-time analysis of living cells.
© 2018 Optical Society of America
Full Article |
PDF Article
More Like This
References
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription
Figures (6)
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription
Tables (1)
You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription
Equations (5)
You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription