Abstract

We demonstrate a method for quantitative phase (QP) imaging without $2\pi$ ambiguity using a spectral domain phase microscopy system. The method is capable of QP measurement of a large dynamic range with a high sensitivity. We determine an integer multiple of $2\pi$ to correct wrapped phases by calculating the phase shift difference between the detected interference fringe and simulated fringes. The presented method is quantitatively verified by measuring the vibration generated by a piezo linear stage and mapping the surface topography of a slanted mirror. QP imaging of red blood cells is also performed to demonstrate the method’s capacity and application in biological imaging.

© 2018 Optical Society of America

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