Abstract

A combination of multispectral photoacoustic microscopy (PAM) and optical coherence tomography (OCT) by a single light source was previously realized discretely; however, this is unfavorable for visualizing vital physiological and pathological activities in vivo. Here, a co-impulse dual-mode imaging system that simultaneously enables multispectral PAM and OCT using a megahertz supercontinuum pulse laser in vivo is presented. The 500–600 nm band is used for functional PAM imaging, which can flexibly switch between different wavelengths, while the 600–840 nm band is selected for OCT imaging. A mimicking phantom experiment and in vivo imaging of normal and melanoma mouse ears demonstrate that the co-impulse multispectral PAM-OCT system can simultaneously provide structural and functional information for bioimaging.

© 2019 Optical Society of America

Single all-fiber-based nanosecond-pulsed supercontinuum source for multispectral photoacoustic microscopy and optical coherence tomography

Xiao Shu, Magalie Bondu, Biqin Dong, Adrian Podoleanu, Lasse Leick, and Hao F. Zhang
Opt. Lett. 41(12) 2743-2746 (2016)

All-optically integrated photo-acoustic microscopy and optical coherence tomography based on a single Michelson detector

Zhongjiang Chen, Sihua Yang, Yi Wang, and Da Xing
Opt. Lett. 40(12) 2838-2841 (2015)

Optically integrated trimodality imaging system: combined all-optical photoacoustic microscopy, optical coherence tomography, and fluorescence imaging

Zhongjiang Chen, Sihua Yang, and Da Xing
Opt. Lett. 41(7) 1636-1639 (2016)

Miniature probe integrating optical-resolution photoacoustic microscopy, optical coherence tomography, and ultrasound imaging: proof-of-concept

Xianjin Dai, Lei Xi, Can Duan, Hao Yang, Huikai Xie, and Huabei Jiang
Opt. Lett. 40(12) 2921-2924 (2015)

Multimodal imaging with spectral-domain optical coherence tomography and photoacoustic remote sensing microscopy

Matthew T. Martell, Nathaniel J. M. Haven, and Roger J. Zemp
Opt. Lett. 45(17) 4859-4862 (2020)