Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Ultrafast pH-jump two-dimensional infrared spectroscopy

Abstract

We present a pH-jump two-dimensional infrared (2D IR) spectrometer to probe pH-dependent conformational changes from nanoseconds to milliseconds. The design incorporates a nanosecond 355 nm source into a pulse-shaper-based 2D IR spectrometer to trigger dissociation of a caged proton prior to probing subsequent conformational changes with femtosecond 2D IR spectroscopy. We observe a blue shift in the amide I mode (CO stretch) of diglycine induced by protonation of the terminal amine. This method combines the bond-specific structural sensitivity of ultrafast 2D IR with triggered conformational dynamics, providing structural access to multiscale biomolecular transformations such as protein folding.

© 2019 Optical Society of America

Full Article  |  PDF Article
More Like This
Transient two-dimensional spectroscopy with linear absorption corrections applied to temperature-jump two-dimensional infrared

Kevin C. Jones, Ziad Ganim, Chunte Sam Peng, and Andrei Tokmakoff
J. Opt. Soc. Am. B 29(1) 118-129 (2012)

Ultrafast nonequilibrium Fourier-transform two-dimensional infrared spectroscopy

Carlos R. Baiz, Matthew J. Nee, Robert McCanne, and Kevin J. Kubarych
Opt. Lett. 33(21) 2533-2535 (2008)

Drop-in two-dimensional electronic spectroscopy based on dual modulation in the pump-probe geometry

Daniel G. Oblinsky, Evgeny E. Ostroumov, and Gregory D. Scholes
Opt. Lett. 44(11) 2653-2656 (2019)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved