Abstract
We present a pH-jump two-dimensional infrared (2D IR) spectrometer to probe pH-dependent conformational changes from nanoseconds to milliseconds. The design incorporates a nanosecond 355 nm source into a pulse-shaper-based 2D IR spectrometer to trigger dissociation of a caged proton prior to probing subsequent conformational changes with femtosecond 2D IR spectroscopy. We observe a blue shift in the amide I mode ( stretch) of diglycine induced by protonation of the terminal amine. This method combines the bond-specific structural sensitivity of ultrafast 2D IR with triggered conformational dynamics, providing structural access to multiscale biomolecular transformations such as protein folding.
© 2019 Optical Society of America
Full Article | PDF ArticleMore Like This
Kevin C. Jones, Ziad Ganim, Chunte Sam Peng, and Andrei Tokmakoff
J. Opt. Soc. Am. B 29(1) 118-129 (2012)
Carlos R. Baiz, Matthew J. Nee, Robert McCanne, and Kevin J. Kubarych
Opt. Lett. 33(21) 2533-2535 (2008)
Daniel G. Oblinsky, Evgeny E. Ostroumov, and Gregory D. Scholes
Opt. Lett. 44(11) 2653-2656 (2019)