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Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens

P. Pozzi, M. Quintavalla, A. B. Wong, J. G. G. Borst, S. Bonora, and M. Verhaegen

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Abstract

In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.

© 2020 Optical Society of America

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Supplementary Material (2)

NameDescription
Code 1       A Python implementation of the DONE optimization algorithm for 64-bit Windows.
Code 2       Interface to perform generic sensorless adaptive optics using Dynamic optics' 18 actuators adaptive lens by optimizing microscopy images directly from the acquisition computer's screen.

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