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Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues

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Abstract

We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.

© 2020 Optical Society of America

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Supplementary Material (4)

NameDescription
Visualization 1       Simultaneous fluorescence lifetime and Raman maps of tissues.
Visualization 2       Fluorescence lifetime phasor maps of tissues, channel 1.
Visualization 3       Fluorescence lifetime phasor maps of tissues, channel 2.
Visualization 4       Processed Raman spectra and maps of tissue.

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