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In vivo measurement of the lineal density of red blood cells in human retinal capillaries using high-speed adaptive optics ophthalmoscopy

Boyu Gu, David Sarraf, Michael Ip, Srinivas R. Sadda, and Yuhua Zhang

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Abstract

We present an automated method for measuring the lineal density of red blood cells (RBCs) in human retinal capillaries using adaptive optics near-confocal ophthalmoscopy (AONCO). The spatiotemporal traces of RBCs flowing in retinal capillaries were extracted from AONCO images, enhanced using the Gabor filter, the vesselness filter, and the Hough transform. A total of 43 capillary segments (each 50 µm long) were analyzed in 12 eyes of 12 subjects, and the measurement error of the automated method was 0.09 cell over 50 µm compared with results obtained by manual counting. Our method provides a tool for assessing RBC spatial distribution in retinal capillaries.

© 2021 Optical Society of America

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Supplementary Material (7)

NameDescription
Code 1       Main function for measuring RBC lineal density
Code 2       Sub-function for measuring RBC lineal density
Data File 1       Red blood cell lineal density within a cardiac circle in a capillary.
Data File 2       Bland-Altman plot of RBC lineal density measured by manual counting (LDm) versus that by the automated algorithm (LDa).
Data File 3       The distribution of RBC lineal density quantified by manual counting and by the automated measurement.
Supplement 1       Supplemental material
Visualization 1       Spatiotemporal RBC trace image for testing

Data Availability

Data underlying the results presented in this paper are available in Data File 1, Ref. [26]; Data File 2, Ref. [27]; and Data File 3, Ref. [28].

26. B. Gu, D. Sarraf, M. Ip, S. R. Sadda, and Y. Zhang, “Red blood cell lineal density within a cardiac circle in a capillary,” figshare (2021), https://doi.org/10.6084/m9.figshare.14773224.

27. B. Gu, D. Sarraf, M. Ip, S. R. Sadda, and Y. Zhang, “Bland-Altman plot of RBC lineal density measured by manual counting (LDm) versus that by the automated algorithm (LDa),” figshare (2021), https://doi.org/10.6084/m9.figshare.14773227.

28. B. Gu, D. Sarraf, M. Ip, S. R. Sadda, and Y. Zhang, “The distribution of RBC lineal density quantified by manual counting and by the automated measurement,” figshare (2021), https://doi.org/10.6084/m9.figshare.14773230.

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Equations (3)

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