Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

F-mode ultraviolet photoacoustic remote sensing for label-free virtual H&E histopathology using a single excitation wavelength

Not Accessible

Your library or personal account may give you access

Abstract

Photoacoustic remote sensing (PARS) is a novel all-optical imaging modality that allows for non-contact detection of initial photoacoustic pressures. Using 266-nm excitation pulses, ultraviolet PARS (UV-PARS) has previously demonstrated imaging contrast for cell nuclei in histological samples with ${\lt}{400\,\,\rm nm}$ resolution. In prior PARS-based imaging schemes, the signal amplitude at an interrogation point was determined by the maximum deflection from the DC scattering signal in response to a pulsed excitation. This method, however, does not take into consideration additional information encoded in the frequency domain of the recorded PARS signals. Here, we present a frequency domain technique called F-mode PARS that can be used to generate images with nuclear and cytoplasmic enhanced contrast, enabling label-free virtual hematoxylin-and-eosin-like microscopy, using only a single excitation wavelength. With F-mode processing, we have been able to demonstrate contrast-to-noise ratios of up to 38 dB between cell nuclei and surrounding cytoplasm, which represents up to a 25-dB improvement over previous implementations of UV-PARS systems.

© 2021 Optical Society of America

Full Article  |  PDF Article
More Like This
Virtual histopathology with ultraviolet scattering and photoacoustic remote sensing microscopy

Nathaniel J. M. Haven, Matthew T. Martell, Brendyn D. Cikaluk, Brendon S. Restall, Ewan McAlister, Sveta Silverman, Lashan Peiris, Jean Deschenes, Xingyu Li, and Roger J. Zemp
Opt. Lett. 46(20) 5153-5156 (2021)

Virtual hematoxylin and eosin histopathology using simultaneous photoacoustic remote sensing and scattering microscopy

Brendon S. Restall, Nathaniel J. M. Haven, Pradyumna Kedarisetti, Matthew T. Martell, Brendyn D. Cikaluk, Sveta Silverman, Lashan Peiris, Jean Deschenes, and Roger J. Zemp
Opt. Express 29(9) 13864-13875 (2021)

Ultraviolet photoacoustic remote sensing microscopy

Nathaniel J. M. Haven, Kevan L. Bell, Pradyumna Kedarisetti, John D. Lewis, and Roger J. Zemp
Opt. Lett. 44(14) 3586-3589 (2019)

Data Availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved