Abstract
Photoacoustic remote sensing (PARS) is a novel all-optical imaging modality that allows for non-contact detection of initial photoacoustic pressures. Using 266-nm excitation pulses, ultraviolet PARS (UV-PARS) has previously demonstrated imaging contrast for cell nuclei in histological samples with ${\lt}{400\,\,\rm nm}$ resolution. In prior PARS-based imaging schemes, the signal amplitude at an interrogation point was determined by the maximum deflection from the DC scattering signal in response to a pulsed excitation. This method, however, does not take into consideration additional information encoded in the frequency domain of the recorded PARS signals. Here, we present a frequency domain technique called F-mode PARS that can be used to generate images with nuclear and cytoplasmic enhanced contrast, enabling label-free virtual hematoxylin-and-eosin-like microscopy, using only a single excitation wavelength. With F-mode processing, we have been able to demonstrate contrast-to-noise ratios of up to 38 dB between cell nuclei and surrounding cytoplasm, which represents up to a 25-dB improvement over previous implementations of UV-PARS systems.
© 2021 Optical Society of America
Full Article | PDF ArticleMore Like This
Nathaniel J. M. Haven, Matthew T. Martell, Brendyn D. Cikaluk, Brendon S. Restall, Ewan McAlister, Sveta Silverman, Lashan Peiris, Jean Deschenes, Xingyu Li, and Roger J. Zemp
Opt. Lett. 46(20) 5153-5156 (2021)
Brendon S. Restall, Nathaniel J. M. Haven, Pradyumna Kedarisetti, Matthew T. Martell, Brendyn D. Cikaluk, Sveta Silverman, Lashan Peiris, Jean Deschenes, and Roger J. Zemp
Opt. Express 29(9) 13864-13875 (2021)
Nathaniel J. M. Haven, Kevan L. Bell, Pradyumna Kedarisetti, John D. Lewis, and Roger J. Zemp
Opt. Lett. 44(14) 3586-3589 (2019)