Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Multimodal nonlinear-optical imaging of nucleoli

Not Accessible

Your library or personal account may give you access

Abstract

Multimodal nonlinear microscopy combining third-harmonic generation (THG) with two- and three-photon-excited fluorescence (2PEF and 3PEF) is shown to provide a powerful resource for high-fidelity imaging of nucleoli and nucleolar proteins. We demonstrate that, with a suitably tailored genetically encoded fluorescent stain, the 2PEF/3PEF readout from specific nucleolar proteins can be reliably detected against the extranucleolar 2PEF/3PEF signal, enabling high-contrast imaging of the key nucleolar ribosome biogenesis components, such as fibrillarin. THG is shown to provide a versatile readout for unstained nucleolus imaging in a vast class of biological systems as different as neurons in brain slices and cultured HeLa cells.

© 2021 Optical Society of America

Full Article  |  PDF Article
More Like This
Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation

M. S. Pochechuev, A. A. Lanin, I. V. Kelmanson, D. S. Bilan, D. A. Kotova, A. S. Chebotarev, V. Tarabykin, A. B. Fedotov, V. V. Belousov, and A. M. Zheltikov
Opt. Lett. 44(12) 3166-3169 (2019)

Cell-specific three-photon-fluorescence brain imaging: neurons, astrocytes, and gliovascular interfaces

A. A. Lanin, M. S. Pochechuev, A. S. Chebotarev, I. V. Kelmanson, D. S. Bilan, D. A. Kotova, V. S. Tarabykin, A. A. Ivanov, A. B. Fedotov, V. V. Belousov, and A. M. Zheltikov
Opt. Lett. 45(4) 836-839 (2020)

Pulse photothermal optical coherence tomography for multimodal hemodynamic imaging

Jiayi Wu, Nanshou Wu, Peijun Tang, Jiayi Lin, Yi Lian, and Zhilie Tang
Opt. Lett. 46(22) 5635-5638 (2021)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Data Availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved