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Structured illumination microscopy for simultaneous imaging of achiral and chiral domains

Jiwei Zhang, Shiang-Yu Huang, Ankit Kumar Singh, and Jer-Shing Huang

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Abstract

We propose double structured illumination microscopy (SIM) method, which enables simultaneous imaging of achiral and chiral domains at sub-wavelength resolution. In double SIM, the illumination field is spatially structured both in the intensity and optical chirality so that moiré effects can be concurrently generated on the achiral and chiral fluorescent domains of a sample. This allows for down-modulating the high spatial frequency of both domains at the same time and thus provides sub-wavelength details after image reconstruction. We introduce the working principle of double SIM and theoretically demonstrate the feasibility of this method using different kinds of synthetic samples.

© 2021 Optical Society of America

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Supplementary Material (1)

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Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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