Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

High-throughput optical sectioning via line-scanning imaging with digital structured modulation

Not Accessible

Your library or personal account may give you access

Abstract

Optical sectioning with high-throughput, a high signal-to-noise ratio (SNR), and submicrometer resolution is crucial, but challenging, to three-dimensional visualization of large biological tissue samples. Here we propose line-scanning imaging with digital structured modulation for optical sectioning. Our method generates images with a significantly improved SNR, compared to wide-field structured illumination microscopy (WF-SIM), without residual modulation artifacts. We image a ${14.5}\;{\rm mm} \times {11.5}\;{\rm mm}$ horizontal view of mouse brain tissue at a pixel resolution of ${0.32}\;\unicode{x00B5}{\rm m} \times {0.32}\;\unicode{x00B5}{\rm m}$ in 101 s, which, compared to WF-SIM, represents a significant improvement on imaging throughput. These results provide development opportunities for high-throughput, high-resolution large-area optical imaging methods.

© 2021 Optical Society of America

Full Article  |  PDF Article
More Like This
High-quality high-throughput silicon laser milling using a 1 kW sub-picosecond laser

Daniel Holder, Rudolf Weber, Christoph Röcker, Gerhard Kunz, David Bruneel, Martin Delaigue, Thomas Graf, and Marwan Abdou Ahmed
Opt. Lett. 46(2) 384-387 (2021)

Single-scan HiLo with line-illumination strategy for optical section imaging of thick tissues

Wei Qiao, Rui Jin, Tianpeng Luo, Yafeng Li, Guoqing Fan, Qingming Luo, and Jing Yuan
Biomed. Opt. Express 12(4) 2373-2383 (2021)

OpenWSI: a low-cost, high-throughput whole slide imaging system via single-frame autofocusing and open-source hardware

Chengfei Guo, Zichao Bian, Shaowei Jiang, Michael Murphy, Jiakai Zhu, Ruihai Wang, Pengming Song, Xiaopeng Shao, Yongbing Zhang, and Guoan Zheng
Opt. Lett. 45(1) 260-263 (2020)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved