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Multispectral compressive fluorescence lifetime imaging microscopy with a SPAD array detector

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Abstract

Multispectral/hyperspectral fluorescence lifetime imaging microscopy ($\lambda$FLIM) is a promising tool for studying functional and structural biological processes. The rich information content provided by a multidimensional dataset is often in contrast with the acquisition speed. In this work, we develop and experimentally demonstrate a wide-field $\lambda$FLIM setup, based on a novel time-resolved $18\times 1$ single-photon avalanche diode array detector working in a single-pixel camera scheme, which parallelizes the spectral detection, reducing measurement time. The proposed system, which implements a single-pixel camera with a compressive sensing scheme, represents an optimal microscopy framework towards the design of $\lambda$FLIM setups.

© 2021 Optical Society of America

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