Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Implementation of a fluorescence spatiotemporal modulation super-resolution microscope

Not Accessible

Your library or personal account may give you access

Abstract

Super-resolution microscopy (SRM) unveils details of subcellular organelles and provides a technical foundation for cellular biology research. Long-term, non-invasive live-cell super-resolution imaging requires low-intensity illumination and high image quality. Here, we present a new, to the best of our knowledge, method based on time-resolved detection termed fluorescence spatiotemporal modulation, in which highly spatially resolved photons in the beam center are extracted by taking the difference of the photons in the beam periphery with a weighted coefficient. The experimental results show a sub-100 nm resolution at tens of microwatts of laser power. Our proposed method requires only one laser, laying a foundation for a lower-cost multi-color super-resolution imaging system.

© 2022 Optica Publishing Group

Full Article  |  PDF Article
More Like This
Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy

Shaocong Liu, Zhimin Zhang, Yubing Han, Lu Yang, Cuifang Kuang, and Xu Liu
Opt. Lett. 46(13) 3304-3307 (2021)

Line-wise scanning-based super-resolution imaging

Xin Tian, Ying Xiao, Rui Liu, Fang He, and Jiayi Ma
Opt. Lett. 47(9) 2230-2233 (2022)

Plug-and-play pixel super-resolution phase retrieval for digital holography

Xuyang Chang, Liheng Bian, Yunhui Gao, Liangcai Cao, Jinli Suo, and Jun Zhang
Opt. Lett. 47(11) 2658-2661 (2022)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved