Abstract
Super-resolution microscopy (SRM) unveils details of subcellular organelles and provides a technical foundation for cellular biology research. Long-term, non-invasive live-cell super-resolution imaging requires low-intensity illumination and high image quality. Here, we present a new, to the best of our knowledge, method based on time-resolved detection termed fluorescence spatiotemporal modulation, in which highly spatially resolved photons in the beam center are extracted by taking the difference of the photons in the beam periphery with a weighted coefficient. The experimental results show a sub-100 nm resolution at tens of microwatts of laser power. Our proposed method requires only one laser, laying a foundation for a lower-cost multi-color super-resolution imaging system.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Shaocong Liu, Zhimin Zhang, Yubing Han, Lu Yang, Cuifang Kuang, and Xu Liu
Opt. Lett. 46(13) 3304-3307 (2021)
Xue Cheng, Qi Li, Yiqun Duan, Yan Chen, Junlin Teng, Saisai Chu, Hong Yang, Shufeng Wang, and Qihuang Gong
Opt. Lett. 48(10) 2655-2658 (2023)
Xihao Zhang, Jing Wang, Simone Lamon, Min Gu, and Qiming Zhang
Opt. Lett. 47(16) 4223-4226 (2022)