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Interferometrical single-molecule localization based on dynamic PSF engineering

Lei Wang, Jing Lu, Wei Ji, Lingyu Wan, and Lusheng Gu

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Abstract

We present a method for interferometric single-molecule localization based on dynamic point spread function (PSF) engineering. By using two galvo mirrors, a hexagonal PSF is constructed and the fluorescent signal under different illumination patterns could be acquired simultaneously. This method was evaluated using simulation, fluorescent nanosphere imaging, and single-molecule imaging. The study indicates a twofold improvement in localization precision while maintaining the same photon budget. This strategy, we believe, is a cost-effective way to increase the resolution of single-molecule localization microscopy.

© 2022 Optica Publishing Group

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Corrections

Lei Wang, Jing Lu, Wei Ji, Lingyu Wan, and Lusheng Gu, "Interferometrical single-molecule localization based on dynamic PSF engineering: publisher’s note," Opt. Lett. 47, 2870-2870 (2022)
https://opg.optica.org/ol/abstract.cfm?uri=ol-47-11-2870

17 May 2022: A typographical correction was made to the author listing.

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Supplementary Material (1)

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Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request. Simulation and analysis code of this paper may be obtained from the authors upon reasonable request.

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