Dual-modality imaging system for monitoring human heart organoids beating in vitro

Senyue Hao; Chao Ren; Chao Ren; Fei Wang; Kibeom Park; Brett D. Volmert; Brett D. Volmert; Aitor Aguirre; Aitor Aguirre; Chao Zhou; Chao Zhou

doi:10.1364/OL.493824

Optics Letters
Vol. 48,
Issue 15,
pp. 3929-3932
(2023)
•https://doi.org/10.1364/OL.493824

Dual-modality imaging system for monitoring human heart organoids beating in vitro

Senyue Hao, Chao Ren, Fei Wang, Kibeom Park, Brett D. Volmert, Aitor Aguirre, and Chao Zhou

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Senyue Hao, Chao Ren, Fei Wang, Kibeom Park, Brett D. Volmert, Aitor Aguirre, and Chao Zhou, "Dual-modality imaging system for monitoring human heart organoids beating in vitro," Opt. Lett. 48, 3929-3932 (2023)

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Table of Contents Category
- Medical Optics and Biotechnology
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About this Article
History
- Original Manuscript: April 20, 2023
- Revised Manuscript: June 14, 2023
- Manuscript Accepted: June 30, 2023
- Published: July 20, 2023

Abstract

To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids (hHOs), we developed a dual-modality imaging system combining the advantages of optical coherence tomography (OCT) and fluorescence microscopy. OCT provides high-resolution volumetric structural information, while fluorescence imaging indicates the electrophysiology of the hHOs’ beating behavior. We verified that concurrent OCT motion mode (M-mode) and calcium imaging retrieved the same beating pattern from the heart organoids. We further applied dynamic contrast OCT (DyC-OCT) analysis to strengthen the verification and localize the beating clusters inside the hHOs. This imaging platform provides a powerful tool for studying and assessing hHOs in vitro, with potential applications in disease modeling and drug screening.

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Supplementary Material (1)

Name	Description
Visualization 1	This video shows concurrently recorded calcium imaging and OCT M-mode imaging videos and corresponding data analysis results from the two ROIs of the same hHO.

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Data presented in this paper are available upon reasonable request to the authors.

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