Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Thin-film optical-acoustic combiner enables high-speed wide-field multi-parametric photoacoustic microscopy in reflection mode

Abstract

Multi-parametric photoacoustic microscopy (PAM) is uniquely capable of simultaneous high-resolution mapping of blood oxygenation and flow in vivo. However, its speed has been limited by the dense sampling required for blood flow quantification. To overcome this limitation, we have developed a high-speed multi-parametric PAM system, which enables simultaneous acquisition of ∼500 densely sampled B-scans by superposing the rapid optical scanning across the line-shaped focus of a cylindrically focused ultrasonic transducer over the conventional mechanical scan of the optical-acoustic dual foci. A novel, to the best of our knowledge, optical-acoustic combiner (OAC) is designed and implemented to accommodate the short working distance of the transducer, enabling convenient confocal alignment of the dual foci in reflection mode. A resonant galvanometer (GM) provides stabilized high-speed large-angle scanning. This new system can continuously monitor microvascular blood oxygenation (sO2) and flow over a 4.5 × 3 mm2 area in the awake mouse brain with high spatial and temporal resolutions (6.9 µm and 0.3 Hz, respectively).

© 2023 Optica Publishing Group

Full Article  |  PDF Article
More Like This
High-speed wide-field multi-parametric photoacoustic microscopy

Fenghe Zhong, Youwei Bao, Ruimin Chen, Qifa Zhou, and Song Hu
Opt. Lett. 45(10) 2756-2759 (2020)

High-speed multi-parametric photoacoustic microscopy of cerebral hemodynamic and metabolic responses to acute hemodilution

Yiming Wang, Fenghe Zhong, Naidi Sun, Zhiqiang Xu, Jun Li, Quan Liu, Zhengying Li, Zhiyi Zuo, and Song Hu
Opt. Lett. 47(8) 1988-1991 (2022)

Fully waterproof two-axis galvanometer scanner for enhanced wide-field optical-resolution photoacoustic microscopy

Jaeyul Lee, Sangyeob Han, Daewoon Seong, Junsoo Lee, Sungjo Park, Ruchire Eranga Wijesinghe, Mansik Jeon, and Jeehyun Kim
Opt. Lett. 45(4) 865-868 (2020)

Supplementary Material (3)

NameDescription
Supplement 1       Supplemental Document
Visualization 1       Dynamic responses of the brain microvascular sO2 and blood flow to hypoxia. Scale bar: 1 mm.
Visualization 2       Dynamic response of the brain microvascular sO2 to hypoxia. Scale bar: 1 mm.

Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved