Abstract

Hyperspectral microscopy (HSM) combines conventional microscopy with basic hyperspectral imaging and results in 3D microscopic spatio-spectral information. The combination comes along with a variety of applications, such as detection and classification of different material properties through spectral fingerprints, which otherwise cannot be detected with a color camera alone. However, similar to other microscopies, the resolution of HSM is limited by diffraction. In recent years microsphere (MS)-assisted microscopy has attracted intensive attention for resolution enhancement. Here, we extend the MS idea into HSM. We show that MS-HSM reveals extra hyperspectral information about the specimen which is lost without it. Moreover, the insertion of MS results in super-resolved images. The resolution enhancement and obtaining the extra hyperspectral information can be tuned by the MS size.

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