Abstract

We present a snapshot temporal compressive light-sheet fluorescence microscopy system to capture high-speed microscopic scenes with a low-speed camera. A deep denoising network and total variation denoiser are incorporated into a plug-and-play framework to quickly reconstruct 20 high-speed video frames from a short-time measurement. Specifically, we can observe 1,000-frames-per-second (fps) microscopic scenes when the camera works at 50 fps to capture the measurement. The proposed method can potentially be applied to observe cell and tissue motions in thick living biological specimens.

© 2023 Optica Publishing Group

Snapshot temporal compressive microscopy using an iterative algorithm with untrained neural networks

Mu Qiao, Xuan Liu, and Xin Yuan
Opt. Lett. 46(8) 1888-1891 (2021)

Snapshot spatial–temporal compressive imaging

Mu Qiao, Xuan Liu, and Xin Yuan
Opt. Lett. 45(7) 1659-1662 (2020)

Snapshot compressive structured illumination microscopy

Runqiu Luo, Miao Cao, Xing Liu, and Xin Yuan
Opt. Lett. 49(2) 186-189 (2024)

Flexible and accurate total variation and cascaded denoisers-based image reconstruction algorithm for hyperspectrally compressed ultrafast photography

Zihan Guo, Jiali Yao, Dalong Qi, Pengpeng Ding, Chengzhi Jin, Yilin He, Ning Xu, Zhiling Zhang, Yunhua Yao, Lianzhong Deng, Zhiyong Wang, Zhenrong Sun, and Shian Zhang
Opt. Express 31(26) 43989-44003 (2023)

Video snapshot compressive imaging using adaptive progressive coding for high-quality reconstruction under different illumination circumstances

Xing Liu, Mingyu Zhu, Siming Zheng, Runqiu Luo, Huazheng Wu, and Xin Yuan
Opt. Lett. 49(1) 85-88 (2024)