Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Issues in Progress Current Issue All Issues Early Posting

Light sheet fluorescence microscopy with active optical manipulation

Jun Yin, Ruijing Liang, Haoyi Hou, Yan Miao, and Lingyao Yu

Not Accessible

Your library or personal account may give you access

Abstract

We present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form a linear optical trapping perpendicular to the optical axis and an excitation light sheet (LS) parallel to the optical axis, respectively. Multiple large-sized polystyrene fluorescent microspheres are stably captured by LOTs, and their rotation angles around specific rotation axes are precisely controlled. During a sample rotation, the stationary excitation LS scans the sample to obtain fluorescence sectioning images of the sample at different angles.

© 2024 Optica Publishing Group

Full Article  |  PDF Article
More Like This
Dynamic speckle illumination wide-field fluorescence microscopy with actively optical manipulation of rotational angles

Jun Yin, Yan Miao, Ruijing Liang, Haoyi Hou, Chuanxin Teng, and Lingyao Yu
Biomed. Opt. Express 15(3) 1585-1594 (2024)

Multimodal high-resolution embryonic imaging with light sheet fluorescence microscopy and optical coherence tomography

Behzad Khajavi, Ruijiao Sun, Harshdeep Singh Chawla, Henry H. Le, Manmohan Singh, Alexander W. Schill, Mary E. Dickinson, David Mayerich, and Kirill V. Larin
Opt. Lett. 46(17) 4180-4183 (2021)

Volume holographic optical element for light sheet fluorescence microscopy

Ting-Yu Hsieh, Sunil Vyas, Jui-Ching Wu, and Yuan Luo
Opt. Lett. 45(23) 6478-6481 (2020)

View More...

Supplementary Material (4)

NameDescription
Visualization 1       The microscopic images of one polystyrene fluorescent microsphere (I) with a diameter of 50 µm in solution under stable capture by LOTs with white arrow presenting moving direction of capillary tube.
Visualization 2       The microscopic images of two polystyrene fluorescent microspheres with diameters of 5 µm under precise manipulation to rotate with LOTs.
Visualization 3       The microscopic images of two polystyrene fluorescent microspheres with diameters of 10 µm under precise manipulation to rotate with LOTs.
Visualization 4       The LSFM fluorescence sectioning images of rotation samples at corresponding angles in the yz plane.

Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use