September 2020
Spotlight Summary by Andrey Kuzmin
Innovative optofluidics and microscopy-based rapid analysis of pathogens
Bacterial sensing is an important technique for detecting pathogenic bacterial infections and preventing the outbreak of illness. Traditional approaches for bacterial infection analysis often includes time-consuming culturing of the bacterium responsible for this infection. On the other hand, optofluidic methods of counting/analysis of microorganisms, which combine the advantages of microfluidics and optics, have become popular due to miniaturization, low power consumption and high sensitivity of this technology for broad applications in chemistry, biology and biomedicine.
An innovative optofluidic technique, developed by the authors of this recent Biomedical Optics Express article, allows rapid pre-concentration of small objects bypassing the need to culture the bacteria. To achieve this goal, the authors propose using a local temperature gradient on the surface of a microscope slide with the biosample, created by a laser beam waist of micron size. This gradient produces convection flow of fluid, which manipulates micro-objects, such as bacteria, to form a population with high concentration.
Additionally, this technique enables controlling the size of manipulated particles by adjusting the power of the laser beam, which, in turn, allows for the selective capture of bacterial cells from different biological samples for optional further antibiotic analysis.
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An innovative optofluidic technique, developed by the authors of this recent Biomedical Optics Express article, allows rapid pre-concentration of small objects bypassing the need to culture the bacteria. To achieve this goal, the authors propose using a local temperature gradient on the surface of a microscope slide with the biosample, created by a laser beam waist of micron size. This gradient produces convection flow of fluid, which manipulates micro-objects, such as bacteria, to form a population with high concentration.
Additionally, this technique enables controlling the size of manipulated particles by adjusting the power of the laser beam, which, in turn, allows for the selective capture of bacterial cells from different biological samples for optional further antibiotic analysis.
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Article Information
Innovative optofluidics and microscopy-based rapid analysis of pathogens
Hao Wang, Priyanka Shiveshwarkar, Robert Brzozowski, Arseny Zhdanov, Shulin Shi, Prahathees Eswara, and Anna Pyayt
Biomed. Opt. Express 11(9) 5060-5069 (2020) View: Abstract | HTML | PDF