Raman quantitative measurements and drug tracking in skin tissue are quite challenging procedures, mostly due to strong background signals, which shadow millimolar concentrations of the investigated compounds. To overcome this problem, the authors of this Biomedical Optics Express publication developed and proved phase-modulated stimulated Raman scattering (PM-SRS) microscopy with a time-resolved signal detection method. PM heterodyne detection allowed the authors to suppress the nonlinear background, while a time-resolved SRS signal detection technique efficiently suppressed Raman signals from the tissue and resulted in better signal contrast of the imaged compound. The imaging contrast of a skin moisturizing agent in an in-vitro skin tissue model for the new method was up to 40 times higher than that, when using the traditional amplitude modulated SRS microscopy. This technique could be efficient for in-vivo monitoring of drug applications in skin diseases.

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