September 2022
Spotlight Summary by Danuta Sampson
Combining fluorescence lifetime with spectral information in fluorescence lifetime imaging ophthalmoscopy (FLIO)
In this paper, the authors show how to combine three different measurements obtained from fluorescence lifetime imaging ophthalmoscopy (FLIO) into one to enhance diagnostic sensitivity for early changes in the retinal pigment epithelium caused by age-related macular degeneration (AMD). AMD is a condition that impacts the middle part of our vision, commonly affecting people in their 50s and 60s.
FLIO delivers fluorescence lifetimes in two spectral channels and the peak emission wavelength (PEW) of the fluorescence. Conventionally, FLIO users look at each parameter separately to reveal information on pathologic alterations. In the current paper, the authors combine these measures into a single parameter – a lifetime-PEW metric. The new approach is validated on a cohort of healthy and AMD individuals and is shown to work well. It enables faster assessment of the image with enhanced sensitivity. More research is required to confirm the applicability of the method to other eye diseases.
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FLIO delivers fluorescence lifetimes in two spectral channels and the peak emission wavelength (PEW) of the fluorescence. Conventionally, FLIO users look at each parameter separately to reveal information on pathologic alterations. In the current paper, the authors combine these measures into a single parameter – a lifetime-PEW metric. The new approach is validated on a cohort of healthy and AMD individuals and is shown to work well. It enables faster assessment of the image with enhanced sensitivity. More research is required to confirm the applicability of the method to other eye diseases.
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Article Information
Combining fluorescence lifetime with spectral information in fluorescence lifetime imaging ophthalmoscopy (FLIO)
Martin Hammer, Rowena Simon, Daniel Meller, and Matthias Klemm
Biomed. Opt. Express 13(10) 5483-5494 (2022) View: Abstract | HTML | PDF