Abstract
We present a light emitting diode (LED) based spectroscopic method for application in highly scattering materials. Using an LED as a light source, frequency domain measurement and the diffusion model of photon migration, we measured the absorption spectrum of a chromophore (methylene blue) suspended in a highly scattering medium (1.54% solids content Liposyn fat emulsion). The resulting spectrum agrees both qualitatively and quantitatively with standard spectropho-tometric measurements obtained without the scattering. Finally, we enumerate reasons why LED-based frequency-domain techniques are very appropriate for medical applications.
© 1994 Optical Society of America
PDF ArticleMore Like This
Michael S. Patterson and Brian W. Pogue
APMPDWI.96 Advances in Optical Imaging and Photon Migration (BIOMED) 1994
Jee H. Choi, Martin Wolf, Ursula Wolf, Chiara Polzonetti, Larisa P. Safonova, Rajarsi Gupta, Antonios Michalos, William Mantulin, Enrico Gratton, Vladislav Toronov, and Dennis Hueber
SuD33 Biomedical Topical Meeting (BIOMED) 2002
Seung Yup Lee, Corey Zheng, and Erin Buckley
JTu3A.58 Clinical and Translational Biophotonics (Translational) 2018