Abstract
The low-fouling and functionalizable surface platforms were investigated in terms of functionalization effect on fouling resistance from undiluted media (blood plasma, food extracts), surface capacities, and biorecognition capabilities. The platforms included hydroxy- and carboxy-functional polymer brushes and standard OEG-based alkanethiolate chemistry. In contrast to hydroxy-functional brushes, the fouling properties of carboxy-functional poly(carboxybetaine acrylamide) (pCBAA) were found to be rather insensitive to functionalization. An SPR sensor combining pCBAA with functionalized gold nanoparticles was capable of detecting Salmonella sp. and E.coli O157:H7 in hamburger samples at concentrations as low as tens of cells/ml.
© 2014 Optical Society of America
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