Abstract
Laser microbeams for irradiation at the subcellular level have been utilized for many years for numerous diverse applications, such as gene transfer, selective chromosome damage, selective cell injury and killing, fluorescence studies, cell-cell communication, and cell fusion.1-4 We report on methods to control damage to cellular components outside the laser focal point as well as give damage threshold values for cellular components as a function of pulse length and beam geometry.
© 1989 Optical Society of America
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