Abstract
We present a novel technique, based on phase-sensitive flow cytometric principles,1,2 that probes the excited state lifetimes of fluorochrome-labeled cells in flow simultaneously at more than one modulation frequency. It allows one to distinguish, in real time, between single-exponential and nonexponential decays for each cell at a high speed (hundreds to thousands of cells per second).
© 1995 Optical Society of America
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