Frequency content of the double-helix PSF for 3D microscopy in the presence of spherical aberration

Sreya Ghosh; Chrysanthe Preza

doi:10.1364/COSI.2011.CTuB3

Imaging and Applied Optics
OSA Technical Digest (CD) (Optica Publishing Group, 2011),
paper CTuB3
•https://doi.org/10.1364/COSI.2011.CTuB3

Frequency content of the double-helix PSF for 3D microscopy in the presence of spherical aberration

Sreya Ghosh and Chrysanthe Preza

Computational Optical Sensing and Imaging 2011

Toronto Canada
10–14 July 2011
ISBN: 978-1-55752-914-5

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PSF Engineering and Pupil Encoding (CTuB)

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S. Ghosh and C. Preza, "Frequency content of the double-helix PSF for 3D microscopy in the presence of spherical aberration," in Imaging and Applied Optics, OSA Technical Digest (CD) (Optica Publishing Group, 2011), paper CTuB3.

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Abstract

We examine the Fourier content of the double helix point-spread function (DH-PSF) by computing the DH optical transfer function (OTF). DH-OTFs are compared to OTFs of conventional fluorescence microscopy in the presence of spherical aberration.

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