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Simultaneous cell morphometry and refractive index measurement with dual-wavelength Digital Holographic Microscopy

Benjamin Rappaz, Florian Charrière, Tristan Colomb, Christian Depeursinge, Pierre J. Magistretti, and Pierre Marquet

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Abstract

The refractive index and cellular thickness information contained in the phase signal recorded by Digital Holographic Microscopy is measured independently with a dual-wavelength technique exploiting the dispersion of a dye present in the perfusion medium.

© 2008 Optical Society of America

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