Application of 3D Tracking, Multi-Wavelength Techniques and Color Imaging in Digital Holographic Microscopy

Björn Kemper; Patrik Langehanenberg; Sebastian Kosmeier; Xiaoli Mo; Xiaoli Mo; Sabine Przibilla; Angelika Vollmer; Steffi Ketelhut; Jinghui Xie; Gert von Bally

doi:10.1364/DH.2009.JMB5

Advances in Imaging
OSA Technical Digest (CD) (Optica Publishing Group, 2009),
paper JMB5
•https://doi.org/10.1364/DH.2009.JMB5

Application of 3D Tracking, Multi-Wavelength Techniques and Color Imaging in Digital Holographic Microscopy

Björn Kemper, Patrik Langehanenberg, Sebastian Kosmeier, Xiaoli Mo, Sabine Przibilla, Angelika Vollmer, Steffi Ketelhut, Jinghui Xie, and Gert von Bally

Digital Holography and Three-Dimensional Imaging 2009

Vancouver Canada
26–30 April 2009
ISBN: 978-1-55752-871-1

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DH/OTA Joint Session (JMB)

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B. Kemper, P. Langehanenberg, S. Kosmeier, X. Mo, S. Przibilla, A. Vollmer, S. Ketelhut, J. Xie, and G. von Bally, "Application of 3D Tracking, Multi-Wavelength Techniques and Color Imaging in Digital Holographic Microscopy," in Advances in Imaging, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper JMB5.

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Abstract

It is demonstrated that digital holographic microscopy (DHM) permits label-free 3D tracking of multiple cells without mechanical focus realignment. Furthermore, by using multi-wavelength techniques in DHM, a significant reduction of amplitude and phase noise is achieved. Finally, results obtained by color DHM prospect subsequent refocusing of color images in the analysis of stained tissue sections.

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