Point spread function model for microscopic image deconvolution in digital holographic microscopy

Anca Marian; Etienne Cuche; Christian Depeursinge

doi:10.1364/ECBO.2003.5143_202

Novel Optical Instrumentation for Biomedical Applications
Proc. SPIE (Optica Publishing Group, 2003),
paper 5143_202
•https://doi.org/10.1364/ECBO.2003.5143_202

Point spread function model for microscopic image deconvolution in digital holographic microscopy

Anca Marian, Etienne Cuche, and Christian Depeursinge

European Conference on Biomedical Optics 2003

Munich Germany
ISBN: 0-8194-5013-8

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Imaging Tools and Methods II (ITMII)

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A. Marian, E. Cuche, and C. Depeursinge, "Point spread function model for microscopic image deconvolution in digital holographic microscopy," in Novel Optical Instrumentation for Biomedical Applications, A. Boccara, ed., Vol. 5143 of Proc. SPIE (Optica Publishing Group, 2003), paper 5143_202.

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Abstract

A simple model is proposed in order to evaluate the complex amplitude point spread function (intensity and phase) of a microscope objective. The model is based on the Fresnel diffraction theory and takes also into account the possible optical aberrations. Experimental evaluation of this amplitude point spread function has been carried out by using a holographic set-up and 60 nanometers gold spheres as punctual objects. The measured values are then compared with the theoretical predicted model.

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