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Conferences > ECBO > 2005 > SuC > SuC3

Measurement of Intracellular Oxygen Levels using Fluorescence Lifetime Imaging Microscopy (FLIM)

Dhruv Sud, Wei Zhong, and Mary-Ann Mycek

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Abstract

We developed a controlled-environment FLIM system towards intracellular oxygen sensing, with a resolution of approximately 8 μM, covering the entire physiologic range (0-300 μM). We measured oxygen levels in normal and cancerous living cells.

© 2005 Optical Society of America

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