Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy

Karla Balaa; Viviane Devauges; Yannick Goulam; Vincent Studer; Sandrine Lévêque-Fort; Emmanuel Fort

doi:10.1364/ECBO.2009.7367_10

Advanced Microscopy Techniques
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2009),
paper 7367_10
•https://doi.org/10.1364/ECBO.2009.7367_10

Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy

Karla Balaa, Viviane Devauges, Yannick Goulam, Vincent Studer, Sandrine Lévêque-Fort, and Emmanuel Fort

European Conference on Biomedical Optics 2009

Munich Germany
14–18 June 2009
ISBN: 9780819476432

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LOCALIZATION AND HIGH PRECISION (LHP)

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K. Balaa, V. Devauges, Y. Goulam, V. Studer, S. Lévêque-Fort, and E. Fort, "Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy," in Advanced Microscopy Techniques, P. Campagnola, E. Stelzer, and G. Von Bally, eds., Vol. 7367 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2009), paper 7367_10.

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Abstract

We present a new imaging technique using surface-plasmon mediated fluorescence microscopy. It uses a similar configuration as standard prismless Total Internal Reflection Fluorescence Microscopy with an additional metallic thin film. In the case of a silver thin film we show that this technique offers many advantages: distance dependence emission filter for improved signal to noise ratio and enhanced molecular detection efficiency. This technique is of particular interest in membrane and adhesion imaging. We present real time images on live cells.

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