Abstract
A rapid and easy method for the monitoring of both live and dead bacteria in a sample is valuable in many fields of microbiological and pharmacodynamics studies, and for the monitoring of food safety and public health. Efficient, culture-independent detection of live and dead bacteria can be achieved using differentially staining fluorescent dyes SYTO 9 and propidium iodide (PI). Fluorescence microscopy and flow cytometry have been used extensively for detection of these live/dead cell fluorescence signals, however, both these methods require bulky equipment and are relatively expensive to implement.
We are optimising a method to determine live and dead bacterial concentration that takes advantage of an inexpensive fibre-based fluorimeter, the optrode, which can measure fluorescence intensity in bacterial solutions in challenging working environments. The concentrations of live and dead bacteria were predicted using multivariate analysis of optrode-measured fluorescence spectra, which is then compared with results obtained from the flow cytometry measurements. The findings from this study will be used to establish a general method for the monitoring of live and dead bacteria using the optrode.
© 2019 SPIE/OSA
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