Abstract

Time-resolved fluorescence spectra can directly reveal changes in electronic state. However, experiments can be difficult when the spectra exhibit overlapping fluorescence profiles because of the inhomogeneous broadening in the energy transfer from DNA to different intercalated dye molecules, for example. The advantage of Raman spectra is the finger-print character, which provides a powerful method for identifying the species involved in the dynamical processes. Therefore, we have performed the multiplex ps CSRS to examining the feasibility for monitoring the vibrational decay times from different species simultaneously. This method combines the advantage of time resolved CSRS^[1] and multiplex CSRS,^[1] which provides an unambiguous identification of multiple species and their dynamical processes. This technique is particularly useful for the study of molecular relaxation and energy trasfer processes.

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