Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Separating Reflective and Fluorescent Components using High Frequency Illumination in the Spectral Domain

Not Accessible

Your library or personal account may give you access

Abstract

Hyperspectral imaging is beneficial to many applications but current methods do not consider fluorescent effects which are present in everyday items ranging from paper, to clothing, to even our food. Furthermore, everyday fluorescent items exhibit a mix of reflectance and fluorescence. So proper separation of these components is necessary for analyzing them. In this paper, we demonstrate efficient separation and recovery of reflective and fluorescent emission spectra through the use of high frequency illumination in the spectral domain. With the obtained fluorescent emission spectra from our high frequency illuminants, we then present to our knowledge, the first method for estimating the fluorescent absorption spectrum of a material given its emission spectrum. Conventional bispectral measurement of absorption and emission spectra needs to examine all combinations of incident and observed light wavelengths. In contrast, our method requires only two hyperspectral images. The effectiveness of our proposed methods are then evaluated through a combination of simulation and real experiments. We also demonstrate an application of our method to synthetic relighting of real scenes.

© 2014 Japan Society of Applied Physics, Optical Society of America

PDF Article
More Like This
A Bilinear Model for Hyperspectral Fluorescence and Reflectance Imaging

Naoyuki Ohara, Yinqiang Zheng, Tomoya Nakamura, Imari Sato, and Masahiro Yamaguchi
30aOD3 Optics and Photonics Japan (OPJ) 2017

High spatial frequency structured light imaging negates absorption effects in fluorescence imaging

Jonathan T. Elliott, David M. McClatchy, Stephen C. Kanick, Kristian J. Sexton, Keith D. Paulsen, Venkataramanan Krishnaswamy, and Brian W. Pogue
BW1B.5 Biomedical Optics (BIOMED) 2014

Molecular Imaging Using Time Domain Fluorescence

Anand T. N. Kumar
BM4B.1 Biomedical Optics (BIOMED) 2014

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.