Abstract

The femtosecond laser amplifier has received much attention as a promising tool for three-dimensionally processing and manipulating micro-sized objects. Especially the focused laser pulse with high peak intensity generates shockwave and stresswave effectively. We applied these waves as an impulsive force to evaluate surface elasticity of biological materials such as zebrafish embryos and biomimetic micro gel-sphere [1,2]. The responses of the mechanical vibration are monitored by the motion of the sample-attached atomic force microscopy (AFM) cantilever. This advanced technique enables sensitive detection of surface and internal elastic properties of biological materials. Herein, we have applied it to elucidate mechanical vibration propagation properties in protein microcrystals and evaluated the result in terms of Young’s modulus estimated from the AFM force curve measurements.

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