Quantitative Second-harmonic Generation Imaging of Tissue Damage from Environmental-scanning Electron Microscopy

Woowon Lee; Woowon Lee; Kimani C. Toussaint; Kimani C. Toussaint; Kimani C. Toussaint

doi:10.1364/FIO.2018.JTu2A.119

Frontiers in Optics / Laser Science
OSA Technical Digest (Optica Publishing Group, 2018),
paper JTu2A.119
•https://doi.org/10.1364/FIO.2018.JTu2A.119

Quantitative Second-harmonic Generation Imaging of Tissue Damage from Environmental-scanning Electron Microscopy

Woowon Lee and Kimani C. Toussaint

Laser Science 2018

Washington, DC United States
16–20 September 2018
ISBN: 978-1-943580-46-0

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Poster Session I (JTu2A)

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W. Lee and K. C. Toussaint, "Quantitative Second-harmonic Generation Imaging of Tissue Damage from Environmental-scanning Electron Microscopy," in Frontiers in Optics / Laser Science, OSA Technical Digest (Optica Publishing Group, 2018), paper JTu2A.119.

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Abstract

We quantitatively evaluate changes of collagenous tissue due to beam exposure in an environmental-scanning electron microscope. Quantitative second-harmonic generation microscopy is used to image and assess selected regions before and after exposure.

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