Abstract
The CLARITY method was used to transform core needle biopsies from patients with breast cancer, into optically transparent tissues, followed by multiplexed immunostaining and 3D imaging of key biomarkers. This data was compared to conventional FFPE thin-section methods. Manual pathology scoring of the 3D images from CLARITY processed samples demonstrated concordant results when compared to a set of 2D images scored throughout a similar sized FFPE block from the same patient.
© 2018 The Author(s)
PDF ArticleMore Like This
David D. Nolte, Ran An, Michael Childress, and John Turek
ATh1O.6 CLEO: Applications and Technology (CLEO:A&T) 2014
Albert E. Cerussi, Vaya W. Tanamai, Darren Roblyer, Shigeto Ueda, Amanda F. Durkin, Rita S. Mehta, David Hsiang, John Butler, and Bruce J. Tromberg
FME1 Frontiers in Optics (FiO) 2010
David D. Nolte, Zhe Li, Honggu Choi, John Turek, and Shadia Jalal
CTu4B.7 Clinical and Translational Biophotonics (Translational) 2018