High-throughput, Label-free and Slide-free Histological Imaging by Ultraviolet-excited Autofluorescence

Yan Zhang; Lei Kang; Xiufeng Li; Terence; T. W. Wong

doi:10.1364/TRANSLATIONAL.2020.JW3A.24

Biophotonics Congress: Biomedical Optics 2020 (Translational, Microscopy, OCT, OTS, BRAIN)
OSA Technical Digest (Optica Publishing Group, 2020),
paper JW3A.24
•https://doi.org/10.1364/TRANSLATIONAL.2020.JW3A.24

High-throughput, Label-free and Slide-free Histological Imaging by Ultraviolet-excited Autofluorescence

Yan Zhang, Lei Kang, Xiufeng Li, Terence, and T. W. Wong

Microscopy Histopathology and Analytics 2020

Washington, DC United States
20–23 April 2020
ISBN: 978-1-943580-74-3

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Joint Poster Session II (JW3A)

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Y. Zhang, L. Kang, X. Li, Terence, and T. W. Wong, "High-throughput, Label-free and Slide-free Histological Imaging by Ultraviolet-excited Autofluorescence," in Biophotonics Congress: Biomedical Optics 2020 (Translational, Microscopy, OCT, OTS, BRAIN), OSA Technical Digest (Optica Publishing Group, 2020), paper JW3A.24.

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Abstract

We proposed a high-throughput label-free and slide-free imaging technique by ultraviolet-excited autofluorescence, assisted by speckle illumination and a deep learning algorithm, to generate subcellular-resolution histology-like images of unprocessed tissues, simplifying the workflow of standard-of-care histopathology.

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