Abstract
We report the use of green fluorescent protein (GFP) as a thermal nanoprobe suited for intracellular temperature mapping. Temperature probing is achieved by monitoring the fluorescence polarization anisotropy of GFP. The method is tested on GFP-transfected cancer cell lines enabling a spatial resolution of 300 nm and a temperature accuracy of 0.4°C [1]. The technique has recently been extended to measure intracellular temperature in an in vivo model of transfected C Elegans.
© 2013 Optical Society of America
PDF ArticleMore Like This
Ja-Yun Lee, Chia-Yun Kao, Ying-Ju Chen, Tzong-Yuan Wu, and I-Jen Hsu
6626_5 European Conference on Biomedical Optics (ECBO) 2007
Ken Abe, Lingling Zhao, Xavier Intes, and Margarida Barroso
QTu3G.5 Quantitative Medical Imaging (QMI) 2013
K. Suhling, D.M. Davis, D. Phillips, J. Siegel, S. Lévêque-Fort, S.E.D. Webb, and P.M.W. French
WD1 Biomedical Topical Meeting (BIOMED) 2002