Abstract
We present a rigid miniaturized optical system block fiber-optic two-photon endoscope based on a compact two-axis piezo scanner system and a miniature high (0.65) NA GRIN lens objective. The optical system is scanned as a whole by a piezo scanner allowing always an on-axis beam irradiation of the optical system. A photonic crystal fiber is used for excitation and ultrashort laser pulses can be delivered with typical power up to at . Two-photon fluorescence signal is collected by the use of a multimode fiber. Lateral resolution values for the system were experimentally measured to be vertically and horizontally. Axial resolution was found to be . The endoscope is highly flexible and controllable in terms of time acquisition, resolution, and magnification. Fluorescence images were acquired over a field of view. Results presented here demonstrate the ability of the system to resolve subcellular details and the potential of the technology for in vivo applications.
© 2009 Optical Society of America
Full Article | PDF ArticleMore Like This
R. Le Harzic, M. Weinigel, I. Riemann, K. König, and B. Messerschmidt
Opt. Express 16(25) 20588-20596 (2008)
Siddharth Sivankutty, Esben Ravn Andresen, Rosa Cossart, Géraud Bouwmans, Serge Monneret, and Hervé Rigneault
Opt. Express 24(2) 825-841 (2016)
Youbo Zhao, Hiroshi Nakamura, and Robert J. Gordon
Biomed. Opt. Express 1(4) 1159-1172 (2010)